- Interim, first-in-human clinical results from initial monotherapy dose-escalation cohorts in ongoing Phase 1/1b study of WTX-124 including safety and preliminary antitumor activity to be described in poster presentation.
- Additional posters representing an expansive body of data demonstrating the potential of Werewolf’s PREDATOR™ platform and INDUKINE™ product candidates will also be presented.
- Company to host conference call and webcast to review WTX-124 initial clinical results November 3, 2023, at 8:30 am ET
WATERTOWN, Mass., Oct. 31, 2023 (GLOBE NEWSWIRE) -- Werewolf Therapeutics, Inc. (the “Company” or “Werewolf”) (Nasdaq: HOWL), an innovative biopharmaceutical company pioneering the development of conditionally activated therapeutics engineered to stimulate the body’s immune system for the treatment of cancer, today announced that a poster describing interim first-in-human clinical results from initial monotherapy dose-escalation cohorts in the ongoing Phase 1/1b study of WTX-124 will be presented at the Society for Immunotherapy of Cancer’s (SITC) 38th Annual Meeting, taking place November 1-5, 2023 in San Diego, California. Additional posters with preclinical data supporting the PREDATOR platform and INDUKINE product candidates will also be presented at the meeting.
“At SITC, we look forward to sharing first-in-human clinical results from our lead candidate, WTX-124, including initial assessments of safety, pharmacokinetics, relevant biomarkers and preliminary antitumor activity from the ongoing monotherapy dose-escalation portion of our Phase 1/1b study in solid tumors. These data will provide early insight into the profile of WTX-124 and preferential activation through our INDUKINE design, including additional data since the abstract submission in June,” said Randi Isaacs, M.D., Chief Medical Officer of Werewolf. “Several preclinical abstracts were also accepted for poster presentation, collectively reinforcing distinct immune activating potential of our INDUKINE molecules across various mechanisms, including IL-2, and as a complement to other anti-cancer approaches, including checkpoint inhibitors and cell therapy.”
All posters will be available at https://investors.werewolftx.com/news-and-events/scientific-resources at 12:00 pm ET on Friday, November 3, 2023. The posters corresponding to the first three abstracts described below will be presented at SITC on Friday November 3rd and the posters corresponding to the second three abstracts described below will be presented on Saturday November 4th.
Highlights of the abstracts, which are now available on the SITC website, include:
Abstract Title: A Phase 1/1b Study of the Tumor-Activated IL-2 Prodrug WTX-124 Alone or in Combination with Pembrolizumab in Patients with Immunotherapy-Sensitive Locally Advanced or Metastatic Solid Tumors Abstract Number: 737
- This Phase 1/1b, multi-center, open-label clinical trial is designed to evaluate WTX-124 as a monotherapy and in combination with KEYTRUDA® (pembrolizumab) in patients with immunotherapy sensitive advanced or metastatic solid tumors who have failed standard of care, including prior checkpoint inhibitor therapy.
- As of June 22, 2023, 11 patients with relapsed/refractory solid tumors, including non-small cell lung cancer, cutaneous melanoma and renal cell carcinoma were treated with WTX-124 in three monotherapy dose escalation cohorts of 1, 3 and 6 mg administered intravenously every two weeks.
- WTX-124 was well-tolerated with no dose limiting toxicities at doses up to 6mg.
- Pharmacokinetic data as of June 22, 2023, demonstrated WTX-124 sustained prodrug exposure in plasma with low levels of active IL-2.
- These results support the potential of WTX-124 to deliver a potent, wild-type IL-2 to the tumor microenvironment in patients with solid tumors with limited toxicities.
Abstract Title: Spatial Analysis of Tumor Infiltrating Lymphocyte Populations in Syngeneic Mouse Tumor Models After Treatment with IL-12 (mWTX-330) and IL-2 (WTX-124) INDUKINETM MoleculesAbstract Number: 1059
- Tumor growth over time was measured in mice bearing syngeneic tumors treated with either mWTX-330 (a chimeric IL-12 containing INDUKINE TM molecule) or WTX-124 (a human IL-2 containing INDUKINE molecule) using various techniques, including high-plex immunofluorescence, resulting in significant remodeling of immune cell populations found within the tumor tissue and simultaneously increased immune cell infiltration generating a potent activation of effector cells.
- These results were further amplified in combination with PD-1 pathway inhibitors, highlighting the potential for INDUKINE TM treatments to improve the effects of checkpoint inhibition therapies.
Abstract Title: Development of WTX-712, a Conditionally Activated IL-21 INDUKINETM Molecule for the Treatment of CancerAbstract Number: 1075
- Human IL-21 receptor knock-in (hIL-21R KI) mice bearing syngeneic tumors were treated with WTX-712, an IL-21 INDUKINETM molecule, or half-life extended human IL-21 to monitor tumor growth and body weight over time via flow cytometry, tissue pharmacokinetics and high-plex immunofluorescence.
- WTX-712 exhibited activity with an expanded therapeutic window compared to half-life extended IL-21 in mouse syngeneic tumor models including complete regressions and protection against tumor growth upon rechallenge.
Title: The Combination of ACT and INDUKINETM Therapy Leads to Improved Antitumor Immunity in Solid TumorsAbstract Number: 252
- The ability of INDUKINE molecules to improve the engraftment and antitumor activity of adoptive cell therapy (ACT) products was evaluated using a pmel-1 transgenic mouse model and a human CD19 CAR-T cell model.
- The combination of pmel-1 ACT and INDUKINETM polypeptides enhanced antitumor activity and animal survival compared to either pmel-1 or INDUKINE treatment alone, including increased donor cell engraftment and persistence of long-term effector memory T cells in both the periphery and the tumor microenvironment.
Title: Optimal Antitumor Immunity Triggered by WTX-124, a Clinical Stage Conditionally Activated INDUKINETM Molecule that Releases Fully Potent IL-2 in the Tumor MicroenvironmentAbstract Number: 1058
- The potential benefits of full activation of IL-2 were evaluated to determine antitumor response in syngeneic tumor models of WTX-124 as compared to non-alpha forms of IL-2 designed to reduce dose limiting toxicities associated with current cytokine therapy.
- Systemic administration of WTX-124 resulted in robust antitumor immunity and preferentially activated tumor-infiltrating immune cells as compared to a non-alpha IL-2 version of the INDUKINE demonstrating that the full activity of IL-2 contained in WTX-124 is required to activate potent antitumor responses.
Title: PK/RO Modeling of WTX-124, a Tumor-Activated IL-2 Prodrug, Highlights the Potential for a Substantially Improved Therapeutic Index Compared to Other IL-2 MoleculesAbstract Number: 1074
- A pharmacokinetic model was developed to evaluate peripheral and tumor lymphocyte IL-2 receptor occupancy for tumor-activated IL-2 molecules such as WTX-124 as compared to non-alpha IL-2 molecules.
- WTX-124 was found to be more likely to improve the therapeutic index by maximizing receptor occupancy on tumor-infiltrating CD8+ T cells than comparable doses of non-alpha IL-2 molecules and substantially higher doses of the non-alpha IL-2 molecule were required to attain the same receptor occupancy.
Conference Call & Webcast Details
Werewolf management will host a conference call and webcast at 8:30 am ET on Friday, November 3, 2023, to review initial clinical results from the ongoing Phase 1/1b study of WTX-124 that will be presented at SITC. The event can be accessed live at https://investors.werewolftx.com/news-and-events/events. An archived replay will be available for approximately 90 days following the event.
About Werewolf Therapeutics:
Werewolf Therapeutics, Inc. is an innovative clinical-stage biopharmaceutical company pioneering the development of therapeutics engineered to stimulate the body’s immune system for the treatment of cancer. We are leveraging our proprietary PREDATOR™ platform to design conditionally activated molecules that stimulate both adaptive and innate immunity with the goal of addressing the limitations of conventional proinflammatory immune therapies. Our INDUKINE™ molecules are intended to remain inactive in peripheral tissue yet activate selectively in the tumor microenvironment. Our most advanced product candidates, WTX-124 and WTX-330, are systemically delivered, conditionally activated Interleukin-2 (IL-2), and Interleukin-12 (IL-12) INDUKINE molecules for the treatment of solid tumors. WTX-124 is in development as a monotherapy and in combination with KEYTRUDA® (pembrolizumab) in multiple solid tumor types. WTX-330 is in development as a single agent in refractory and/or immunotherapy unresponsive or resistant advanced or metastatic solid tumors and non-Hodgkin lymphoma.
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